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1610730 sds  (Bio-Rad)


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    Structured Review

    Bio-Rad 1610730 sds
    1610730 Sds, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1610730+sds/Urea/pm41895286-799-27-35
    Average 96 stars, based on 1145 article reviews
    1610730 sds - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: Analysis of GRK2 aggregation in the pathology of Alzheimer disease in animal models.
    Article Snippet: .. MRC (Medical Research Center), Faculty of Medicine, Cairo, Egypt Ain Shams University Hospitals, Cairo, Egypt Chemicals, peptides, and recombinant proteins Urea electrophoresis purity reagent Bio-Rad Cat. No. 1610730 SDS (Sodium Dodecyl Sulfate) electrophoresis purity reagent Bio-Rad Cat. No. 1610302 Dithiothreitol (DTT) Bio-Rad Cat. No. 161-610 Iodoacetamide Bio-Rad Cat. No. 1632109 Coomassie® Brilliant Blue R 250 Serva 17525 Aqua-Poly/Mount Polysciences, Inc. Cat. No. 1860620 Poly-Mount Xylene Polysciences, Inc. Cat. No. 24176120 Mayer’s hemalum solution Merck KGaA Cat. No. 1.09249.0500 DAPI solution (1 mg/mL) Thermo Fisher Scientific Cat. No. 62248 Amicon® Ultra Centrifugal Filter, 3kDa MWCO Merck Millipore; Sigma-Aldrich Cat. No. UFC8003 Protease Inhibitor Cocktail Sigma-Aldrich Cat. No. P8340 Protein phosphatase inhibitor cocktail Sigma-Aldrich Cat. No. PPC1010 (Continued on next page) e2 Cell Reports Medicine 7, 102707, April 21, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Ni-NTA Agarose Qiagen Cat. No. 30210 PD-10 desalting columns packed with Sephadex G-25 resin GE Healthcare GmbH Cat. No. 17-0851-01 Sephadex TM LH-20 Cytiva Cat. No. 17-0090-01 Zeba TM spin desalting columns (7K MWCO) Thermo Fisher Scientific Cat. No. 89889 Pierce TM Iodination Reagent (1,3,4,6-tetrachloro3α,6α-diphenyl-glycoluril) (Iodo-Gen®) Pierce; Thermo Fisher Scientific Prod.

    Electrophoresis:

    Article Title: Analysis of GRK2 aggregation in the pathology of Alzheimer disease in animal models.
    Article Snippet: .. MRC (Medical Research Center), Faculty of Medicine, Cairo, Egypt Ain Shams University Hospitals, Cairo, Egypt Chemicals, peptides, and recombinant proteins Urea electrophoresis purity reagent Bio-Rad Cat. No. 1610730 SDS (Sodium Dodecyl Sulfate) electrophoresis purity reagent Bio-Rad Cat. No. 1610302 Dithiothreitol (DTT) Bio-Rad Cat. No. 161-610 Iodoacetamide Bio-Rad Cat. No. 1632109 Coomassie® Brilliant Blue R 250 Serva 17525 Aqua-Poly/Mount Polysciences, Inc. Cat. No. 1860620 Poly-Mount Xylene Polysciences, Inc. Cat. No. 24176120 Mayer’s hemalum solution Merck KGaA Cat. No. 1.09249.0500 DAPI solution (1 mg/mL) Thermo Fisher Scientific Cat. No. 62248 Amicon® Ultra Centrifugal Filter, 3kDa MWCO Merck Millipore; Sigma-Aldrich Cat. No. UFC8003 Protease Inhibitor Cocktail Sigma-Aldrich Cat. No. P8340 Protein phosphatase inhibitor cocktail Sigma-Aldrich Cat. No. PPC1010 (Continued on next page) e2 Cell Reports Medicine 7, 102707, April 21, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Ni-NTA Agarose Qiagen Cat. No. 30210 PD-10 desalting columns packed with Sephadex G-25 resin GE Healthcare GmbH Cat. No. 17-0851-01 Sephadex TM LH-20 Cytiva Cat. No. 17-0090-01 Zeba TM spin desalting columns (7K MWCO) Thermo Fisher Scientific Cat. No. 89889 Pierce TM Iodination Reagent (1,3,4,6-tetrachloro3α,6α-diphenyl-glycoluril) (Iodo-Gen®) Pierce; Thermo Fisher Scientific Prod.

    Protease Inhibitor:

    Article Title: Analysis of GRK2 aggregation in the pathology of Alzheimer disease in animal models.
    Article Snippet: .. MRC (Medical Research Center), Faculty of Medicine, Cairo, Egypt Ain Shams University Hospitals, Cairo, Egypt Chemicals, peptides, and recombinant proteins Urea electrophoresis purity reagent Bio-Rad Cat. No. 1610730 SDS (Sodium Dodecyl Sulfate) electrophoresis purity reagent Bio-Rad Cat. No. 1610302 Dithiothreitol (DTT) Bio-Rad Cat. No. 161-610 Iodoacetamide Bio-Rad Cat. No. 1632109 Coomassie® Brilliant Blue R 250 Serva 17525 Aqua-Poly/Mount Polysciences, Inc. Cat. No. 1860620 Poly-Mount Xylene Polysciences, Inc. Cat. No. 24176120 Mayer’s hemalum solution Merck KGaA Cat. No. 1.09249.0500 DAPI solution (1 mg/mL) Thermo Fisher Scientific Cat. No. 62248 Amicon® Ultra Centrifugal Filter, 3kDa MWCO Merck Millipore; Sigma-Aldrich Cat. No. UFC8003 Protease Inhibitor Cocktail Sigma-Aldrich Cat. No. P8340 Protein phosphatase inhibitor cocktail Sigma-Aldrich Cat. No. PPC1010 (Continued on next page) e2 Cell Reports Medicine 7, 102707, April 21, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Ni-NTA Agarose Qiagen Cat. No. 30210 PD-10 desalting columns packed with Sephadex G-25 resin GE Healthcare GmbH Cat. No. 17-0851-01 Sephadex TM LH-20 Cytiva Cat. No. 17-0090-01 Zeba TM spin desalting columns (7K MWCO) Thermo Fisher Scientific Cat. No. 89889 Pierce TM Iodination Reagent (1,3,4,6-tetrachloro3α,6α-diphenyl-glycoluril) (Iodo-Gen®) Pierce; Thermo Fisher Scientific Prod.



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    Peptide binding to PDI mutants Δ252–277 and Δ222–302 is reduced, compared with wild-type. 125 I-labeled glycosylation acceptor peptide, N -benzoyl-NYT-amide, was translocated into PDI wild-type or mutant microsomes as described in Materials and Methods and cross-linked with longwave UV light. All samples were analyzed in duplicate. Proteins were resolved on 7.5% <t>SDS</t> gels and peptide–cross-linking products were visualized by autoradiography. Peptide binding to PDI was quantitated using a <t>PhosphorImager</t> <t>(BioRad).</t>
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    Peptide binding to PDI mutants Δ252–277 and Δ222–302 is reduced, compared with wild-type. 125 I-labeled glycosylation acceptor peptide, N -benzoyl-NYT-amide, was translocated into PDI wild-type or mutant microsomes as described in Materials and Methods and cross-linked with longwave UV light. All samples were analyzed in duplicate. Proteins were resolved on 7.5% <t>SDS</t> gels and peptide–cross-linking products were visualized by autoradiography. Peptide binding to PDI was quantitated using a <t>PhosphorImager</t> <t>(BioRad).</t>
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    Image Search Results


    Peptide binding to PDI mutants Δ252–277 and Δ222–302 is reduced, compared with wild-type. 125 I-labeled glycosylation acceptor peptide, N -benzoyl-NYT-amide, was translocated into PDI wild-type or mutant microsomes as described in Materials and Methods and cross-linked with longwave UV light. All samples were analyzed in duplicate. Proteins were resolved on 7.5% SDS gels and peptide–cross-linking products were visualized by autoradiography. Peptide binding to PDI was quantitated using a PhosphorImager (BioRad).

    Journal: The Journal of Cell Biology

    Article Title: Export of a Cysteine-Free Misfolded Secretory Protein from the Endoplasmic Reticulum for Degradation Requires Interaction with Protein Disulfide Isomerase

    doi:

    Figure Lengend Snippet: Peptide binding to PDI mutants Δ252–277 and Δ222–302 is reduced, compared with wild-type. 125 I-labeled glycosylation acceptor peptide, N -benzoyl-NYT-amide, was translocated into PDI wild-type or mutant microsomes as described in Materials and Methods and cross-linked with longwave UV light. All samples were analyzed in duplicate. Proteins were resolved on 7.5% SDS gels and peptide–cross-linking products were visualized by autoradiography. Peptide binding to PDI was quantitated using a PhosphorImager (BioRad).

    Article Snippet: At the end of the incubation, samples were precipitated with trichloro-acetic acid and analyzed after electrophoresis on 18% polyacrylamide, 4 M urea SDS gels with a BioRad PhosphorImager.

    Techniques: Binding Assay, Labeling, Glycoproteomics, Mutagenesis, Autoradiography